Fibroblast growth factors induce hepatic tumorigenesis post radiofrequency ablation

Fibroblast growth factors induce hepatic tumorigenesis post radiofrequency ablation

Blog Article

Abstract Image-guided radiofrequency ablation (RFA) is Panini Grills / Sandwich Presses used to treat focal tumors in the liver and other organs.Despite potential advantages over surgery, hepatic RFA can promote local and distant tumor growth by activating pro-tumorigenic growth factor and cytokines.Thus, strategies to identify and suppress pro-oncogenic effects of RFA are urgently required to further improve the therapeutic effect.Here, the proliferative effect of plasma of Hepatocellular carcinoma or colorectal carcinoma patients 90 min post-RFA was tested on HCC cell lines, demonstrating significant cellular proliferation compared to baseline plasma.Multiplex ELISA screening demonstrated increased plasma pro-tumorigenic growth factors and cytokines including the FGF protein family which uniquely and selectively activated HepG2.

Primary mouse and immortalized human hepatocytes were then subjected to moderate hyperthermia in-vitro, mimicking thermal stress induced during ablation in the peri-ablational normal tissue.Resultant culture medium induced proliferation of multiple cancer cell lines.Subsequent non-biased protein array revealed that these hepatocytes subjected to moderate hyperthermia also excrete a similar wide spectrum of growth factors.Recombinant FGF-2 activated multiple cell lines.FGFR inhibitor significantly reduced liver tumor load post-RFA in MDR2-KO inflammation-induced HCC mouse model.

Thus, Liver RFA can induce tumorigenesis via the FGF signaling pathway, and its Stirrup Irons inhibition suppresses HCC development.